|
KMID : 0545120120220101432
|
|
Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1432 ~ p.1440
|
|
|
Proteomic Analysis of Proteins of Weissella confusa 31 Affected by Bile Salts
|
|
Lee Kang-Wook
Lee Seung-Gyu
Han Nam-Soo
Kim Jeong-Hwan
|
|
|
Abstract
|
|
|
|
Weissella confusa 31, an isolate from human feces, possesses desirable properties as a probiotic strain, including bile salt resistance. W. confusa 31 is not inhibited by bile salts up to 0.3% concentration. Proteins affected by bile salts (0.05%) were examined by 2-D gel electrophoresis. Our proteomic analyses revealed that the intensities of 29 spots were changed, where 17 increased (including 2 spots observed only under the bile salts stress conditions) and 12 decreased. Proteins were identified by MALDI-TOF mass spectrometry. Proteins increased in the band intensities included adenylate kinase (12.75-fold increase), Clp-like ATP-dependent protease (11.91-fold), 6-phosphogluconate dehydrogenase (10.35- fold), and HSP 70 (5.07-fold). Some of the increased or decreased proteins are also known to be involved in other types of stress responses.
|
|
|
KEYWORD
|
|
|
Weissella, probiotics, bile salts resistance, stress responses, 2-D gel
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|